Prof. Dr. Ingrid Fleming

Goethe University, Institute for Vascular Signalling, Centre for Molecular Medicine

 

Cystathionine gamma lyase and protein S-sulfhydration

 

The Institute for Vascular Signalling (Vascular Research Centre, Frankfurt) is interested in all aspects of signal transduction taking place in the vascular wall under physiological and pathophysiological conditions. Ongoing projects are focused on endothelial cells, monocytes and neutrophils as well as platelets and their interaction with the vasculature. The Institute also has a long tradition of studying the physiological effects of endogenously generated gasotransmitters nitric oxide and hydrogen sulphide (H2S). Currently our group is studying the regulation of the H2S generating enzyme cystathionine g lyase (CSE), CSE-related effects on the vasculature and heart and the role of sulphur metabolites in cell differentiation. Projects make use of modern proteomics techniques to detect protein S-sulfhydration in biological samples to identify the cellular and plasma “sulphydromes”, study the functional consequences of the modification as well as their usefulness as sulphur – containing biomarkers of disease.

For information on additional projects please see our website (www.vrc.uni-frankfurt.de)

 

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Modified Biotin Switch assay followed by proximity ligation assay for the detection of S-Sulfhydrated protein of interest (herein HuR) in murine carotids. Sulfhydration is observed in endothelial cell and smooth muscle cell cytosol on the right panel.